平臺簡介


                            分子成像平臺簡介

                            上?吉利彩票网址?萍即髮W生命學院分子成像平臺主要為生命學院、上??萍即髮W乃至其他研究單位的科研工作者提供高效率、高質量的光學顯微鏡技術支撐服務,為廣大用戶提供生物成像相關的技術和軟件培訓,旨在建成國內設備先進、儀器使用率高和產出效率高的公共實驗室吉利彩票网址。

                            目前平臺已有多臺高級成像設備,主要包括Leica STED超高分辨率共聚焦顯微鏡、Nikon A1R單光子激光共聚焦顯微鏡、Zeiss LSM 710 NLO雙光子激光共聚焦顯微鏡、Zeiss轉盤共聚焦顯微鏡、Olympus VS120快速切片掃描儀、PALM MicroBeam顯微切割系統等。此外,平臺還擁有專業的圖像分析工作站,用戶可使用工作站上的Imaris吉利彩票网址、Volocity和Image J進行數據處理和分析。

                            本成像平臺目前可提供多種成像的技術,具體如下:

                            1. 固定標本或活體的高分辨率成像;

                            2. 光切片成像、三維重建和拼圖。

                            3. 腦片或者其他厚樣品的雙光子成像。

                            4. 長時間活細胞序列成像吉利彩票网址。

                            5. FRET吉利彩票网址、FRAP、光激活和細胞內重要離子濃度檢測等。

                            6. 細胞團吉利彩票网址、單細胞及活細胞等微小樣本的切割和捕獲。

                            7. H&E彩色成像。

                            8. 整張Slide成像,并進行自動無縫式拼圖。

                            9. 圖像處理和分析:反卷積、共定位分析、顆粒追蹤和計數等。


                            Introduction of Molecular Imaging Core Facility


                            Molecular Imaging Core Facility of SLST is focusing on establishing a public platform with the advanced, high efficient equipment. The platform mainly provides the optical microscope technical support and professional training for the users of SLST, whole ShanghaiTech University, and also other research communities.

                            At present, our imaging core has been equipped with several advanced imaging devices, including the Nikon A1R single photon confocal microscope, Zeiss LSM 710 NLO two-photon microscope, Zeiss Spinning Disk confocal microscope, Olympus VS120 slice scanner, PALM laser microdissection systems, etc. In addition, there is a workstation on the platform for image processing and analysis using Imaris, Volocity and Image J.

                            And the platform currently offers a variety of imaging technologies, as follows:

                            1) High-resolution imaging.

                            2) Optic section, 3D reconstruction and stitching large image.

                            3) Two-photon imaging of brain slices or other thick samples.

                            4) Longtime live cell imaging.

                            5) FRET, FRAP, photoactivation and intracellular ion detection.

                            6) Laser capture microdissection.

                            7) H & E slides imaging.

                            8) Whole slide imaging.

                            9) Image processing and analysis: deconvolution, colocalization analysis, particle tracking and counting.



                            吉利彩票网址